Scott Rogers' side haven't played since January 6, when they produced a brilliant performance to beat Yeovil Town Ladies FC 8-0.
The Reds are performing well in the Women's Super League and are currently in third place.
Read on for all of the details ahead of tonight's game...
Where is the game being played? The match is at the Select Security Stadium in Widnes, with kick-off set for 5.30pm GMT.
How can I watch? We will have highlights on LFCTV in the coming week. If you plan to attend the game, click here for ticket details.
View from the captain, Gemma Bonner
"We know how good a team Bristol City are. They play good football and try and play in the right way. They are also a young team with a lot of energy about them. They beat us in pre-season, so we know we will have to be on top of our game and put in a good performance if we want to get the three points. If we can do that then I’m confident we can get the result we want.
"As always, we want to make our fantastic supporters happy and proud. I know the men’s first-team are playing at home on the same night, but we appreciate all the support we get and we know our fans will be singing their hearts out as they always do."