Steven Gerrard's side secured qualification for the last 16 of the competition with a game to spare, after the superb 4-0 away win in Sevilla.
The Reds are confirmed Group E winners and they have joined the likes of Barcelona and Manchester City in the last 16.
Here's our preview of Wednesday's meeting with Spartak Moscow at Prenton Park.
Where is the game being played? The match will take place at Prenton Park, home of Tranmere Rovers FC. Kick-off is set for 12.30pm GMT.
How can I watch the game? We are showing this game live in the UK only on LFCTV GO with our single camera coverage. LFCTV GO UK based subscribers can click here to watch the game. Click here to sign up now.
The story so far: Gerrard's U19s are confirmed Group E winners with four victories and one defeat, 2-1 to Spartak Moscow. The young Reds have scored 16 goals in five games and conceded just three. Second placed Spartak Moscow are already confirmed in the play-offs when they will have a chance to join Liverpool in the last 16.
View from the manager, Steven Gerrard
"We have had a good mix of players for the U19 group with the U18 squad plus the likes of George Johnston, Conor Masterson, Herbie Kane, obviously Ben Woodburn and Rhian Brewster.
"I think the likes of Ben, Rhian and the other players are actually enjoying being around the group, being around their own friends, and I think they also realise that the UEFA Youth League, the club take it very, very serious.
"I think if you're bright as a player, you think, ‘Hang on, the manager has released me to go and play for the U19s here, the manager must be taking it serious’.
“So if Jürgen's taking it serious, why shouldn't it be serious for these players? Because he's watching the games, he gets a report on the games as well, so it's a good opportunity for them.
"Our focus now turns to this game with Spartak Moscow in the UEFA Youth League on Wednesday and hopefully we can get a positive result in our final group game."